The WRKY transcription factors family gene NtWRKYY2（GenBank: KX056496）were cloned by PCR technology from Narcissus tazetta var. ‘Yunxiang’. The Open Reading Frame (ORF) of NtWRKYY2 contained 867 bp, encoding a protein of 289 amino acid residues. Multiple alignments and phylogenetic analysis showed that NtWRKYY2 proteins containing one WRKY consecutive structural domain and C₂H₂ type zinc finger（Csx4Cx23HxH）belong to Ⅱd subgroup of WRKY transcription factor together. Quantitative realtime PCR (qRTPCR) analysis demonstrated that the NtWRKYY2 gene was obviously induced by salt stress in ‘Yunxiang’. We constructed overexpressing vector pMDC140NtWRKYY2 using InFusion cloning technique, and transformed into tobacco by the method of Agrobacterium though leaf disc transformation. The carrier of PCR results indicated that the resistant plantlets were positive and the converting rate of 72%. Salt stress and chlorophyll fluorescence parameter analysis showed that after the salt stress treatment, overexpression of NtWRKYY2 transgenic tobacco showed less wilt and chlorosis than the wild type plants, and the decrease of F_v/F_m value was less than the wild type plants. Studies have shown that overexpression of the NtWRKYY2 gene remarkably increased the salt tolerance in transgenic tobacco. This study offers an alternative gene for salt tolerant transgenic breeding of Narcissus.