In this study, Prunus salicina Lindli. var. cordata J.Y.Zhang et al was chosen as experiment material, the dilution pool PCR method was used to screen a normalized fulllength cDNA library of P. salicina var. cordata leaves and to obtain 12 the fulllength cDNA of WRKY homologous genes, which are named PsWRKY7, PsWRKY14, PsWRKY20, PsWRKY21, PsWRKY22, PsWRKY29, PsWRKY31, PsWRKY32, PsWRKY33, PsWRKY40, PsWRKY46 and PsWRKY75, respectively. Their length are 1 517, 1 798, 2 098, 1 177, 1 242, 1 396, 2 135, 1 760, 2 113, 1 047, 1 258 and 700 bp, respectively. Their ORF lengths are respective 11 095, 1 572, 1 764, 1 068, 1 071, 1 002, 1 944, 1 602, 1 770, 963, 1 077 and 672 bp. They are encoded as 364, 523, 587, 355, 357, 333, 647, 533, 589, 320, 358 and 223 amino acids, respectively. The results of Real Time PCR showed that PsWRKY expression levels increased significantly after spraying with 1.0mmol/L salicylic acid. This result indicated that PsWRKY might be important regulatory factor for inducing plant defense response by salicylic acid.