In order to identify the function of pqqE and GDH genes of Pseudomonas sp. GZJR8 isolated from Hylomecon japonica in the case of dissolved phosphorus, we knocked out pqqE and GDH genes by the homologous recombination technology, marked as ΔpqqE and ΔGDH, and we constructed the complementary strain by electro transformation, marked as ΔpqqE(pqqE) and ΔGDH(GDH). The results of qualitative analysis with insoluble inorganic phosphorus medium (PKO) showed that ΔpqqE and ΔGDH could not produce dissolved phosphorus ring, ΔpqqE(pqqE) and ΔGDH(GDH) could produce dissolved phosphorus ring, compared with the wild type strain (WT). The total amount of available phosphorus produced by WT, ΔpqqE, ΔpqqE(pqqE), ΔGDH and ΔGDH(GDH) was 1 939.000 mg/L, 1 279.000 mg/L, 1 999.000 mg/L, 439.000 mg/L and 2 314.000 mg/L, respectively. Compared with WT, ΔpqqE decreased by 1.52 times, ΔpqqE(pqqE) increased by 1.03 times, ΔGDH decreased by 4.42 times, and ΔGDH(GDH) increased by 1.19 times. The pH of the supernatant after centrifugation of the culture medium showed that the pH of WT, ΔpqqE, ΔpqqE(pqqE), ΔGDH and ΔGDH(GDH) was 4.08, 4.34, 4.03, 4.71 and 4.00, respectively. Compared with WT, ΔpqqE increased by 1.06 times, ΔpqqE(pqqE) decreased by 0.27 times, ΔGDH increased by 1.15 times, ΔGDH(GDH) decreased by 1.02 times. The research showed that pqqE and GDH genes had the ability to dissolve phosphorus, in which ΔpqqE和ΔGDH were lower than WT, but they did not completely lose it, while the complementary strains could restore the ability. The Pseudomonas sp. GZJR8 isolated from Hylomecon japonica could dissolve phosphorus through the acidproducing mechanism. It had potential application value in agricultural production.