In order to study the cell structure and nutrient accumulation characteristics during megaspore and female gametophyte development in Lycium barbarum, we used semithin sectioning and histochemical methods in this study. The main results were as follows: (1) in L. barbarum the ovules attached to the central axis of the ovary which has more locules, and ovules are of anatropous, simple integument and thin nucellus; (2) archesporial cell can directly develop into megaspore mother cell. After meiosis the megasporocyte divided into linear type tetrads. The megaspore in the chalazal end developed into functional one. The development of embryo sac belongs to the polygonum type. The mature embryo sac is surrounded by integument tapetum; (3) in the early period, there were almost no nutrients accumulated in peripheral tissue, but a lot of starch grains appeared in the mature embryo sac, and there are more starch grains in micropylar end than in the chalazal end. The mature embryo sac was composed of an egg apparatus, one central cell with secondary nucleus, 3 antipodal cells; (4) the synergid contained obvious filiform apparatus, which had PAS positive reaction, presented the nature of the polysaccharide. At the mature embryo sac stage, some nucellar cells at the chalazal region differentiated into hypostase.