In this study，the high generation potato strains (342 and 201011) were used as experiment materials. The experiment mainly studies the impact of PVY and PVS complex infection on the P_max(maximum net photosynthetic rate),AQE(apparent quantum efficiency),Cond(stomatal conductance),R_d(dark respiration rate),T_r(transpiration rate),I_c(light compensation point),C_i (intercellular carbon dioxide concentration) and I_sat(light saturation point), and the potato qualities consist of tuber yield, dry matter contents, starch contents, reducing sugar contents and crude protein contents. The results shown that: (1) PVS content in leaves of 201011 is significantly lower than that of 342, the PVY content in leaves of 201011 is lower than that of 342 too, but not significantly different. (2) The average yield of per plant of 342 significantly decreased by 48.54%, while 201011 slightly decreased by 19.98% after infection with PVY and PVS complex virus. The dry matter contents reduced separately by 6.9% and 0.66%, respectively. The starch respectively decreased 8.06% and 3.38%, but not significantly different. However, the crude protein contents increased significantly by 14.05% and 29.17%, while the contents of reducing sugar decreased significantly by 11.11% and 14.29%. The dry matter contents of 342 significantly reduced by 6.9%,while 201011 reduced by 0.66%, but not significantly different. In photosynthetic parameters，SPAD (soil and plant analyzer development) values significantly reduced by 13.37% and 20.1%, respectively. P_max significantly decreased by 32.48% and 4.54%，but other photosynthetic parameters were not significantly changed. This study suggests that the complex infection with PVY and PVS can decrease the contents of SPAD which affects the photosynthesis. Virus infection simultaneously restrained reducing sugar and starch contents in tubers. However, the infection promotes the accumulation of crude protein in tubers.