Six E. sibiricus accessions from Northeast Margin of QinghaiTibet Plateau were used as materials in this study. We evaluated the seed shattering degree at different developmental stages by breaking tensile strength (BTS), and analyzed the correlation between shattering degree, agronomic traits and geographic information. It could provide some conferences for seed production and screening of low seed shattering germplasm in E. sibiricus. The results showed that: (1) E. sibiricus possessed the lower seed shattering degree at WAH1 and WAH2, shattering degree increased at WAH3 and WAH4. Ultimately, seed shattering degree reached the highest at WAH5 (BTS=0.52 N); ZHN03 had lower seed shattering degree at all tested stages when compared with other accessions, and XH09 had the highest seed shattering degree at WAH5 (BTS=0.33 N). (2) Six agronomic traits of E. sibiricus with low seed shattering and high seed shattering had similar changes during the seed shattering; Seed shattering had close relationships with 5 agronomic traits in E. sibiricus. BTS was significantly positive correlation with panicle length, number of spikelet and number of floret per spikelet, and highly significantly positive correlation with awn length and thousand kernel weight. (3) Panicle length of E. sibiricus had a significantly negative correlation to latitude; Awn length was significantly negative correlation with latitude; Thousand kernel weight had a highly significantly negative correlation to longitude, and had a highly significantly positive correlation to altitude; BTS was significantly positive correlation with number of spikelet, and highly significantly positive correlation with awn length and number of floret per spikelet, but had no significant correlation with geographical distribution. (4) The materials in this study were clustered into three clusters according to agronomic traits. Cluster Ⅰ (HZ02, LT04 and XH09) had high seed shattering. Cluster Ⅱ (MQ01 and LQ03) had medium seed shattering. Cluster Ⅲ, ZHN03, had the lowest seed shattering. The study found that seed shattering of E. sibiricus increased along with seed maturation; The seed shattering degree varied with different materials and seed developmental stages, and all accessions had significant difference at WAH4 and WAH5; Seed shattering of E. sibiricus had close correlation with its panicle length, number of spikelet, awn length, thousand kernel weight and and number of floret per spikelet.