Several new records were found in the process of the forth national survey on Chinese material medica resources of Shennongjia National Nature Reserve in Hubei from 2011 to 2013. Among these species, we make a report on Brassicaceae in this article, which include three new record genera and its corresponding three new record species. Megadenia pygmaea Maximowicz belongs to Megadenia Maximowicz and Neotorularia humilis(C. A. Meyer) Hedge & J. Léonard belongs to Neotorularia Hedge & J. Léonard, Sisymbrium luteum(Maximowicz) O. E. Schulz belongs to Sisymbrium Linnaeus. At the same time, we make an analysis on its geographical distribution. Not only can it enrich our knowledge on these three species, but also it can help us study plant diversity and floristic composition in Hubei.