The genetic diversity of 6 wild Dactylis glomerata accessions contained 90 individuals was analysis with AFLP technique. (1) A total of 208 polymorphic bands were amplified with 4 primer pairs, thus the percentage of polymorphism was 59.86% and polymorphism information content was 0.205 3. The Neis genetic diversity varied from 0.201 9 to 0.233 6, which total and average were 0.335 8 and 0.230 9, respectively. The Shannon information index was from 0.235 5 to 0.293 5, which total and average were 0.399 4 and 0.266 4, respectively. (2) The UPGMA trees for individuals and accessions, STRUCTURE analysis and principal coordinate analysis indicated that materials which sampling from same region were clustered together, revealing distinct regionalism. AMOVA revealed 63.30% of genetic differentiation was occurred within accessions and 36.70% was among accessions, while the variance of two sampling regions was 23.51%. The tested germplasm collections revealed abundant genetic variability and high level of differentiation, and population genetic diversity was affected by not only geographical isolation, but also environmental factors like altitude, temperature and monthly precipitation.