In order to investigate the effects of different promoters on the resistance function of GhCDPK1 in cotton, we cloned two promoter sequences of Arabidopsis thaliana RD29A with length of 824 bp and 1 524 bp, and constructed the GhCDPK1 fusion expression vector driven by 35S promoter and two RD29A promoters, respectively. These expression vectors were introduced into wild type tobacco by Agrobacteriummediated leaf disk transformation method. After the drought, salt and low temperature treatments, the tobacco was analyzed through the changes of phenotypic and the content of chlorophyll, peroxidase activity, superoxide dismutase activity, MDA content, proline content and the physiological of membrane permeability. The results indicated that RD29A promoterdriven transgenic tobacco showed stronger resistance than the 35S promoterdriven transgenic tobacco. And the chlorophyll content, proline content, POD and SOD activities were higher than those of 35S promoter driven transgenic tobacco. But MDA content and cell membrane permeability were lower than those of 35S promoter driven tobacco. The GhCDPK1 transgenic tobacco driven by 1 524 bp promoter of RD29A2, was more resistant to stress than transgenic tobacco driven by the RD29A1 promoter of 824 bp.