This study was aimed to isolate the full length cDNA of DoCRT1 in Dendrobium officinale using RACE, followed by bioinformatics analysis. And the expression pattern of the gene was detected by quantitative PCR. The results showed that: (1) the full length cDNA of DoCRT1 (GenBank accession KT957551) was 1 672 bp in length with an ORF of 1 275 bp, encoding a 424 aa protein with a molecular weight of 49.05 kD and an isoelectric point of 4.42. The deduced DoCRT1 protein had the conserved calreticulin/calnexin P domain (205-320), the concanavalin Alike lectin/glucanase domain (18-243), and several motifs. DoCRT1 contained a signal peptide (1-23) and a transmembrane region (8-24) at the N terminus. The protein was predicted to locate predominantly in cell vacuolar and outside, and had high identities (80%-87%) with CRT proteins from various plants. (2) DoCRT1 was closely related to OsCRT1/2 and ZmCRT1, and was clustered to the CRT1/2 Group of the CRT evolutionary tree. (3) DoCRT1 transcripts were more abundant in roots with 2.23 fold over that in leaves, while the expression level of the gene had no apparent difference in stems and leaves. The study will be useful for uncovering molecular role of the gene involving in the growth, development, and physiological stress adaptation in D. officinale.