In this study, PgMYB308，the related gene to the synthesis of lignin, was cloned using RACE and RTPCR methods, with ‘Punica granatum cv. Hongyushizi’ as the experimental materials. The cDNA fulllength sequence of PgMYB308 was 792 bp, which reduced 263 amino acids. Sequence alignment and functional domain analysis revealed that PgMYB308 contained R2, R3, C1, C2, C4 and Zing finger motifs. Phylogenetic tree analysis indicated that PgMYB308 shared the same origin with other species, and had the closest relationship with Eucalyptus grandis EgMYB308. The fluorescent quantitative PCR analysis showed that PgMYB308 expression was detected in stems, leaves and seeds. The expression in stems were the highest, while the lowest in leaves; PgMYB308 expression was higher in ‘Tunisiruanzi’, while lower in ‘Hongyushizi’ and ‘Baiyushizi’; PgMYB308 expression was detected in ‘Hongyushizi’ seeds of different periods. The relative expression of PgMYB308 gradually declined from 20 d to -120 d, and the relative expression was the highest at 20 d.