Study on distribution of PPO gene activity in different wheat ecological region cultivars would benefit screening elite germplasms for improving wheat quality. A total of 379 wheat cultivars were genotyped by the functional markers PPO16, PPO29 and PPO18. The results indicated that: (1) There were two types of PPO genes on 2AL chromosomes: PpoA1a (high PPO) and PpoA1b (low PPO), and their frequency were 51.5% and 48.5%, respectively. (2) there were three types of PPO genes on 2DL chromosomes: PpoD1a (low PPO), PpoD1b (high PPO) and PpoD1ab (intermediate type), and their frequency were 57.8%, 32.5% and 9.8%, respectively. (3)There were six different gene combinations on the chromosome of 2AL and 2DL: PpoA1a/D1a (intermediate type), PpoA1a/D1b (high PPO), PpoA1a/PpoD1ab (intermediate type), PpoA1b/D1a (low PPO), PpoA1b/D1b (intermediate type) and PpoA1b/PpoD1ab (intermediate type). The frequency of PpoA1b/D1a (low PPO) was 25.6%. (4) The distribution frequency about different variation types of wheat PPO gene in various ecological regions existed obvious differences: the proportion of the genotype of PpoA1b was larger in northern winter wheat region and southwestern winter wheat region, and, the proportion of the genotype PpoD1a was larger in Yellow and Huai facultative winter wheat area and northern winter wheat region. The proportion of genotype combination PpoA1b/D1a was larger in northern winter wheat region. Therefore, molecular marker assisted selection (MAS) method could be considered for genetic improvement of appearance quality of wheat grain, and through introducing fine germplasm from the northern winter wheat region.