We cloned a MYB transcription factor named CsMYB123 from the tea plant (Camellia sinensis) cultivar ‘Zijuan’ using RTPCR. Bioinformatics analysis of CsMYB123 gene showed that it contains a 915 bp ORF that encodes a protein with 304 amino acid residues, an estimated molecular weight of 34.07 kD and an isoelectric point of 8.69. Based on an analysis that utilized BLAST online tools, we conclude that CsMYB123 belongs to the MYB gene family and is a R2R3MYB protein. Our molecular evolutionary tree analysis of the CsMYB123 protein and all of the MYB transcription factors in Arabidopsis thaliana indicates that the CsMYB123 protein is most closely related to AtMYB123 and is a member of subgroup 5, which is one of the 22 R2R3MYB subgroups. CsMYB123 is not predicted to have an Nterminal signal peptide or a transmembrane domain. CsMYB123 is predicated to be a hydrophilic protein that is localized to the nucleus. The qPCR results indicated that the expression level of CsMYB123 gene in different tissues was as following: the first leaf and a bud > the second leaf > the third leaf > the fourth leaf > old stem > tender stem, with the expression of CsMYB123 gene in the first leaf and a bud was 15.68 fold higher than that in tender stem；However, expression of CsMYB123 was downregulated under the treatment of IAA, ABA, ETH and GA₃. We detected anthocyanin content in different tissues of ‘ Zijuan’ tea plant, which was listed as below: the second leaf > the first leaf and a bud > the third leaf > the fourth leaf > tender stem > old stem, and the content in the second leaf and the first leaf and a bud were 15 times and 11 times higher than that in old stem, respectively. Based on the study above, we found that CsMYB123 gene was highly expressed in tender shoots of ‘Zijuan’ tea plant, and the expression level of CsMYB123 gene was positively correlated with the content of anthocyanin in different tissues. Thus, CsMYB123 may promote the accumulation of anthocyanins in tea plant.