Using the core fragment screened by mRNA differential display technology in our laboratory preliminary study, which is about 414 bp length deserved from the Conringia planisiliqua L. under drought stress, we cloned the full length cDNA by RACE technology and sequence analysis showed it has higher similarity to AtNSP5, so it was named CpNSP5. The length of CpNSP5 is about 1 228 bp and with an open reading frame of 966 bp. It encodes a protein composed of 321 amino acids whose molecular weight is 35.034 5 kD and the isoelectric point is 5.41. The secondary structure of the CpNSP5 protein contained 26 βsheets and had the Kelch repeat conserved domain. According to phylogentic tree analysis, CpNSP5 kept close relationship with Brassica rapa. Realtime quantitative PCR analysis showed that the transcript of CpNSP5 was induced strongly under 20% PEG6000 stress and 200 mmol·L^-1 NaCl stress. These results indicated that CpNSP5 might be involved in stress responses of C. planisiliqua L. which can provide a candidate gene for crop drought stress breeding.