A CBF1like gene was isolated from Crocus alatavicus Regel et Sem, a perennial ephemeral plant distributed in high mountain of Xinjiang, China, by RTPCR method using a cDNA template. The gene is named as CrCBF1. Sequence analysis indicates that the open reading frame of CrCBF1(GenBank accession MF681 787) gene has 642 bp length, which encoding 213 amino acids with the molecular mass of 23.8 kD and the pI value of 4.99, the CrCBF1 protein encompasses conserved motif of AP2; The result of subcellular localization indicated that CrCBF1 protein was located in the nuclear, the transcriptional activation activity analysis in yeast indicated that CrCBF1 protein harbors the transcriptional activation domain and possess selfactivation ability; The result of function identification in yeast indicated that overexpression of CrCBF1 gene enhance the ability of coldresistance of recombinant yeast.