In this study, PgKO, the gibberellin biosynthesisrelated gene, was cloned from flowers of pomegranate using RTPCR and RACE technology methods, with ‘Punica granatum cv. Hongyushizi’ as the experimental material. Its accession of GenBank is MG208017. The cDNA fulllength sequence of PgKO was 1 729 bp, with 1 542 bp open reading flame encoding 513 amino acids. The protein molecular weight and isoelectric point were 141.16 kD and 4.9 respectively. Multiple alignment showed that PgKO had 61.28%, 64.56% and 73% similarity with Malus × domestica MdKO, Pyrus communis PcKO and Vitis vinifera VvKO respectively, also included CYP450 domain embracing prolinerich domain, redio sites and hemebinding domain. Phylogenetic tree analysis indicated that PgKO shared the same origin with other species, and had the closest relationship with Eucalyptus grandis EgKO. Real time quantitative PCR showed that no matter the bud stage or flowering stage, PgKO expression in cylindricshaped flowers were higher than that in trumpetshaped flowers. Sometimes PgKO expression was detected in ovary, anther and calyx. In cylindricshaped flowers, the expression in ovary is the highest, while lowest in calyx; In trumpetshaped flowers, the expression in anther is the highest, while lowest in ovary.