The fulllength cDNA sequence of the 1deoxyDxylulose5phosphate synthase (DXS) gene from the petals of Lilium Oriental hybrid ‘Entertainer’ was cloned using RACE methods, named as LeDXS (GenBank accession number MF576067). The full length of LeDXS cDNA is 2 471 bp and it contains an open reading frame of 2 142 bp, encoding a putative protein of 713 amino acids. The predicted protein molecular weight and theoretical isoelectric point of LeDXS are 76.3 kD and 6.65, respectively. The formula of LeDXS is C₃₃₇₀H₅₃₇₄N₉₄₂O₁₀₁₆S₃₂. Phylogenetic analysis indicates that LeDXS, clustered with DXS of kiwi fruit and marigold proteins, belongs to the plant DXSⅠcluster and is a functionally conserved protein. LeDXS gene was expressed in the petals of all cultivars based on qRTPCR analysis. LeDXS had a higher expression level in strongscented lilies compared to weakscented and nonscented lilies. The results will benefit our biologically functional understanding of LeDXS, and further research of floral fragrance breeding of lilies.