Abstract:Seven new potato strains (NMSA42, NMSA187, NMSA217, NMSB53, NMSB107, NMSB327 and NMSC92) selected from F1 segregating populations of three potato cross combinations. In order to clarify the differences of main characters, molecular and cellular genetics among them, with potato parental materials as control, we compared and analyzed the traits of the seven new strains, include yield, commercial potato rate, nutritional quality, maturity, PMCM Ⅰ chromosome pairing behavior, pollen fertility and SSR fingerprint characteristics. This will provide the basis for further breeding and registration of new potato varieties. The results showed that: (1) NMSA42 was the new strain of powder processing with high drymatter content, NMSB107 was the new strain with high anthocyanin content, NMSB327 was colored potato new strain, and other new strains were all fresheating potato. (2) The new strain NMSB327 was midearly matured, NMSC92 was latematured, and other strains were midmatured. (3) The average single plant yield of all the strains ranged from 1.02 kg to 1.84 kg, and the commodity potato rate ranged from 81.10% to 94.36%, of which the strain NMSB327 was the highest. The fertile pollen rate ranged from 48.82% to 87.69%, of which the strain NMSA42 was the lowest, and the strain NMSB107 was the highest. (4) The chromosome pairing configuration of the seven new strains NMSA42, NMSA187, NMSA217, NMSB53, NMSB107, NMSB327 and NMSC92 were 6.57Ⅰ+ 9.66Ⅱ + 5.06Ⅲ + 1.74Ⅳ, 5.38Ⅰ+ 8.98Ⅱ + 4.63Ⅲ + 2.69Ⅳ, 4.05Ⅰ + 15.54Ⅱ + 2.12Ⅲ + 1.63Ⅳ, 4.55Ⅰ + 12.12Ⅱ + 2.39Ⅲ + 3.01Ⅳ, 1.93Ⅰ + 11.52Ⅱ + 2.0Ⅲ + 4.26Ⅳ, 4.37Ⅰ + 9.97Ⅱ + 3.19Ⅲ + 3.53Ⅳ and 6.08Ⅰ + 7.6Ⅱ + 3.4Ⅲ + 4.13Ⅳ, respectively. (5) A total of three suitable SSR primers, S25, S118 and S153, which were clear, polymorphic and reproducible, were selected from 55 SSR primers. The SSR fingerprint was constructed by PCR amplification, which could clearly identify 7 new strains and their parents. The study suggested that the seven excellent new potato strains were shallow eyes, good shaped, concentrated and tidy, and the traits of maturity, yield, commercial potato rate and nutritional quality had their own characteristics. They have high application value.