In this study, the fulllength sequence of fructokinase gene (LbFRK7) was obtained by using RTPCR from wolfberry (Lycium barbarum Linn.) ‘Ningqi No.1’ fruit based on TR28373|c0_g1 transcriptome sequencing. The fulllength sequence of LbFRK7 was 1 060 bp （ORF length 1 044 bp）and encoding a polypeptide of 348 amino acids. The relative molecular weight was 37.44 kD and the theoretical isoelectric point (pI) was 5.05. The amino acid sequence of LbFRK7 contained three highly conserved specific regions interent to the pfkB family of carbohydrate kinases, two substrates recognition locus and four ATP binding locus. Homologous genes comparison indicated that LbFRK7 had more high similarity with tobacco and pepper fructokinase genes（90% homology）. The change of LbFRK7 expression was founded in different plant tissues by quantitative realtime PCR. LbFRK7 expressed in the highest in fruits and in the lowest in roots. LbFRK7 expression of fruits showed a trend on firstly rising and then falling during fruit ripening, and reached on the highest level at 15 days after full bloom. LbFRK7 expression had a similar trend with fructose content at the early stage of fruit development, while there was an opposite change between LbFRK7 expression and fructose content at the mid and late stages. LbFRK7 expression was not significantly correlation with fructose and sucrose contents by correlation analysis (r₁=-0.326, r₂=-0.339). Therefore, LbFRK7 played a role on converting fructose during wolfberry fruit development, particularly on increasing of fructose content during the process of fruit ripening. This study provided a reference basis on researching into function of LbFRK7 and metabolism of fructose.