The objective of the present study is exploring the effect of exogenous GA₃ and 6BA on biennial bearing, flowering and branching in “Fuji”. Using 4yearold “Fuji” trees, we sprayed 75 mg/mL GA₃ or 6BA at several different growth periods. we also sprayed 50 mg/mL and 100 mg/mL GA₃ or 6BA at different spraying repeat (single spraying or multiple spraying). The flowering rate, branching rate and endogenous hormones (GA₃, IAA, ABA, ZT) in different types of tree branches were tested. The results showed that: (1) at all growth periods, GA₃ treatment promoted the accumulation of endogenous GA₃ and ABA in terminal buds, but inhibited the synthesis of ZT in all types of branches and IAA in short branches, decreased the flowering rate of different types of branches , promoted branching rate in short branches. 6BA treatment promotes the synthesis of GA₃, ABA, IAA , ZT and flowering rate in all terminal buds of different branches, the branching rate of short shoots, the elongation of long branches also increased. (2) Single spraying of GA₃ inhibited the synthesis of IAA, promoted the accumulation of GA₃, ABA in all branches terminal buds and ZT in short branches. The flowering rate and the elongation of branch were inhibited, the shoot forming rate was promoted by this treatment. the effect of GA₃ on ZT increased with the increase of GA₃ concentration. Multiple spraying of GA₃ increased the accumulation of GA₃ and IAA in all terminal buds, but decreased the content of ABA in the short branches, had no effect on ZT. The long branch increased, the flowering rate and the branch forming rate decreased by this treatment. (3) Single application of 6BA increased the contents of GA₃ and ABA in short terminal buds and ZT content in all branches, but inhibited the synthesis of GA₃ and ABA in long branches and IAA synthesis in all terminal buds. It inhibited the elongation of short branches and promoted the elongation of long branches. Multiple spraying of 6BA increased the contents of GA₃ and IAA in all terminal buds and ZT in short branches, decreased the contents of ABA in all branches and ZT in long branches, and inhibited the flowering rate and elongation of all branches. There was no significant effect on the branching rate and the elongation of long branch. In conclusion, the use of exogenous hormones at different growth stages can promote the synthesis of GA₃ and ABA in the top buds, but the effects of the contents of IAA, ZT, the rate of flower formation, the branching rate and the length of the branches vary with the treatment period. The spraying times and concentrations had different effects on endogenous hormones synthesis, shoot forming rate and branch elongation, but they all inhibited flower bud differentiation and flowering rate.