This study was based on the soybean genome database. According to the amino acid sequence of Arabidopsis thaliana ABI4 protein, we obtained two GmABI4 genes in soybean genome and named GmABI41 (GenBank accession number XM_014766551.1) and GmABI42 (GenBank accession number NM_001249003), respectively. The TMHMM software and phylogenetic analysis showed that the proteins encoded by these two genes had no signal peptides, and their secondary structures mainly consisted of extended strand and random coil. Phylogenetic analysis revealed that soybean GmABI4 was closely related to that of wild soybean. The results of real time quantitative PCR analysis showed that the expression levels of GmABI41 and GmABI42 in seeds and soybean meal were higher than those in roots, stems, leaves, flowers and other tissues, which suggest that GmABI4 is probably involved in seed biology.