We cloned the homologous which was the predicted gene of Brassica napus transcription factor TCP7like, named BnTCP7. The nucleotide sequence similarity of BnTCP7 and Brassica napus TCP7like NC_027757 was 95.68%. The open reading frame was 648 bp, encoding 215 amino acids. The comparison of BnTCP7 with other 19 TCP7 or TCP7like amino acids in Brassicaceaes, homologous proteins were found of TCP7 in B. napus and Raphanus sativus. And the domains have strong similarity and conservatism, especially near the N end with a typical helixloophelix structure. It also showed that BnTCP7 is a hydrophilic unstable protein which belongs to classⅠof TCP family. The online prediction and comparative analysis of secondary and tertiary structures of BnTCP7 and Arabidopsis thaliana AtTCP7 (NC_003076) further revealed that BnTCP7 has the typical structure of TCP family. Phylogenetic analysis indicated that BnTCP7 protein was clustered with Brassica napus (rape) NC_027757 in the same branch, and had the closest relationship. The expression patterns of BnTCP7 gene expression in different organs at different stages were analyzed by the transcriptional data which was found that there was a certain difference in the expression level, and the expression in the vegetative organs was higher than that of the reproductive organs. In addition, the effects of abiotic stresses and hormone treatments on the expression of BnTCP7 gene on the seedling of B. napus were analyzed experimentally. It was found that the BnTCP7 gene not only responds to the abiotic stresses of cold, heat and wound, but also participates in the signals transduction of ABA and GA₃ hormones, which indicated that BnTCP7 might play an important role in maintaining normal growth and development and stresses of plants.