Two auxin response genes named FvARF10 and FvARF18 were cloned from Fragaria vesca using RTPCR. Bioinformatics analysis showed that FvARF10 and FvARF18 contained 2 056 bp and 2 100 bp ORFs, encoding 685 and 699 amino acid residues, respectively. It is predicted that the molecular mass of FvARF10 and FvARF18 proteins were 75.73 kD and 76.78 kD, which isoelectric point were 8.12 and 6.55. Phylogenetic tree analysis indicated that FvARF18 was most closely related to MnARF18 from Morus notabilis, and FvARF10 was most closely related to BnARF10 gene from Boehmeria nivea. FvARF10 and FvARF18 are predicated to be auxin response genes that are located in the nucleus or cytoplasm. Promoter analysis revealed that the sequence of FvARF10 and FvARF18 contained multiple hormone response elements. Results of the realtime PCR analysis showed that FvARF10 and FvARF18 were constitutively expressed in all examined tissues. FvARF18 was strongly expressed in stem, while FvARF10 was strongly expressed in half red fruit. The relative transcript level of FvARF10 and FvARF18 were induced strongly under IAA treatment, and reached the maximum after treated by IAA 1 h. These results indicated that FvARF10 and FvARF18 genes may be involved in the regulation of vegetative reproduction and development of strawberry, which related to the signal transduction of auxin.