In order to investigate the sequence characteristics and expression of VOZ (vascular plant onezinc finger protein) transcription factors in pineapple, we analyzed the sequences of VOZ transcription factors by bioinformatics software. qRTPCR was performed to analyze the expression patterns of VOZ transcription factors in pineapple seedlings under the treatments of NaCl, drought, low temperature (4 ℃), mannitol, Eth, SA, ABA, H₂O₂ and PEG6000. The results showed that: (1) the VOZ transcription factors in pineapple, Arabidopsis thaliana and rice all contained two conserved regions, and protein analysis showed that the AcoVOZ was hydrophilic acid protein. Secondary structure prediction showed that the main structural elements of AcoVOZ protein were alphahelix and random coil. (2) qRTPCR analysis showed that the expression of AcoVOZ1 and AcoVOZ2 were significantly different from the control under different abiotic stress conditions. Among them, the expression levels of AcoVOZ1 and AcoVOZ2 showed a significant decrease under the treatments of NaCl, drought, low temperature (4 ℃), mannitol, ABA, and Eth. The results suggested that VOZ transcription factors in pineapple participate in the response process of pineapple under various abiotic stresses such as highsalt, drought and low temperature (4 ℃), which may be helpful for the further study on molecular mechanisms of pineapple stress resistance and genetic improvement of pineapple resistance.