A population of 262 recombinant inbred lines (RILs) from a fluecured tobacco cross between Y3 and K326 by single seed descent (SSD) method was used to construct a genetic linkage map. 626 SSR loci were evenly mapped into 24 linkage groups (LGs) which spanned a total length of 1 120.45 cM. With a randomized complete block design, one location field tests with three replicates in two years were conducted to characterize seven agronomic traits related to leaf yield, including plant height (PH), internode length (IL), leaf number (LN), stem girth (SG), angle between leaf and stem (ALS), length of waist leaf (LWL) and width of waist leaf (WWL). Based on the data of field trials and rMQM method, we mapped and analyzed the QTLs for seven target characters. The results showed:（1）the seven target traits of fluecured tobacco had a wide range of continuous variation in the RILs population, with significant bidirectional superparental segregation, and the average value of each trait was very close to the midparent value. The average broad heritability of 7 agronomic traits was 73.33%, of which the generalized heritability of PH and IL was above 80%, while the SG and ALS was less than 60%, indicating that 7 agronomic traits related to fluecured tobacco yield were quantitative traits controlled by both microeffects and environmental conditions.（2）A total of 30 QTLs were detected on 9 linkage groups, while five major QTLs with relatively large effects, explaining for more than 10% of the phenotypic variation in the RILs population, related to PH, IL, LN, LWL, and WWL were detected by both two years. （3）The seven traits were largely correlated with each other. Consistent with this, some small regions harboring two or more closely linked QTLs of different traits were found in the genome. The results provide an important theoretical basis for developing high yield cultivars in tobacco through markerassisted selection (MAS).