In order to study the regulatory mechanisms of flavonoid metabolic pathway in Chinese narcissus，we cloned a R2R3MYB gene named NtMYB7 （GenBank accession MF522208）. The sequence analysis showed that NtMYB7 had a 753 bp ORF, encoding 250 amino acids. Amino acid sequence analysis showed that NtMYB7 contained conserved R2 and R3 binding domains. NtMYB7 was grouped with anthocyanin repressors by phylogenetic tree analysis. The expression level of NtMYB7 gene was varied in different organs and different developmental stages of Chinese narcissus and was the highest in basal plates, which was investigated by qRTPCR. Transient expression analysis found NtMYB7 reduced the red pigment induced by the anthocyanin synthesis activating factor StMYB. Quantitative PCR analysis showed that NtMYB7 significantly repressed the expression of tobacco FLS gene which belongs to flavonoid metabolic branch. At the same time, NtMYB7 downregulated the expression of anthocyanin and proanthocyanidin synthesis structural genes activated by StMYB in tobacco leaves. The results preliminarily identify NtMYB7 as a repressor of the flavonoid metabolic pathway in Chinese narcissus.