The NHCC004 of Brassica campestris was used as the experimental material. A pair of primers were designed according to the BcMAF2 gene sequence of NHCC004. The results showed that：（1） BcMAF2 contained an open reading frame（ORF） of 588 bp which encodes 196 amino acids. The comparison of amino acid sequence of BcMAF2 protein with other species showed that the BcMAF2 gene had a highly conserved domain with other species.（2） The subcellular localization vector pEarleyGate101BcMAF2YFPHA was expressed transiently in the leaves of Nicotiana tabacum via Agrobacterium tumefaciensmediated method. The results showed that BcMAF2 protein was located in the nucleus, acting as a transcription factor. （3）The plant expression vector of pEarleyGate101BcMAF2YFPHA and then introduced into Arabidopsis by dipping method and 5 strains of overexpression lines were obtained by Western blot. Late bolting was observed in the line 8 and 10 transgenic Arabidopsis thaliana. This research predicted result shows that BcMAF2 participates in vernalization pathway of flowering in plants.