Gynura divaricata samples were collected from 12 differently experimental fields in Shaanxi Province, and the amount of total flavonoids and B vitamins in stems and leaves were measured. The redundancy analysis (RDA) was conducted to reveal the relationship between active components and differently ecological factors, which were obtained from China Integrated Meteorological Information Service System. The results indicated that: (1)the amount of total flavonoids and B vitamins in G. divaricata from different fields showed significant differences. The total flavonoids in G. divaricata from 4 experimental fields in Luonan County were the highest, whereas the amount of B vitamins was the lowest. Besides that, the content of total flavonoids appeared significant negative correlation with the amount of B2, B₆, B₁₂ and Bvitamins in stems and leaves of G. divaricata. (2) Geographic position and environmental factors, such as elevation, precipitation and local temperature affected the accumulation of active components in G. divaricata. Interestingly, the total flavonoids could be positively determined by elevation and precipitation, but for B vitamins, it presented negative correlation with these two factors. So, it is concluded that Luonan county in the Qinba Mountain Area (alt: 800-900 m; average monthly rainfall in JulAug: 78-84 mm; average ground temperature: 26-27.5 ℃) was an optimal place for the cultivation of G. divaricata in Shaanxi Province.