In July 2016 and August 2017，algae samples were collected twice from TwoRiver Source Natural Reserve of Altay Mountains in Xinjiang. Through microscopic observation，we found five Chinese new record plants，which belong to 4 genera，including 4 species and 1 variety. They were Closterium tumidulum Gay，Pleurotaenium crenulatum (Ralfs) Rabenhorst，Euastrum coeselii Kouwets，Cosmarium crenulatum Ngeli，Cosmarium reniforme var. compressum Nordstedt.