Identification and Expression Analysis of WRKY71 Transcription Factor Family Genes in Brassica juncea
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    Abstract:

    In this study, based on the result of the genome survey sequencing of Brassica juncea, we cloned four WRKY71s genes by RTPCR technology, which named BjuWRKY7114. Bioinformatics analysis showed that BjuWRKY7114 contained 822, 840, 834 and 855 bp ORFs, encoding 273, 279, 277 and 284 amino acid residues, respectively. It is predicted that the molecular mass of BjuWRKY7114 proteins were 30.80, 27.11, 31.58, 32.18 kD, which isoelectric point were 7.75, 9.08, 8.35 and 7.76, respectively. Phylogenetic tree analysis showed that BjuWRKY7114 had the highest similarity with BrWRKY71 transcription factor. Subcellular localization prediction indicated that BjuWRKY71s were localized to the nucleus. BjuWRKY7114 genes were located on the chromosome A09, B04, A07 and scaffold144, respectively. Results of qRTPCR analysis showed that four BjuWRKY71s genes were responsive to high salt, abscisic acid(ABA) and low temperature stress. Under salt stress, the expression of BjuWRKY713 was increased continuously, while the expression of the other three genes was the highest at 6 h, and then decreased. Under the treatment with ABA, the expression of BjuWRKY71s gene was the highest at 6 h and then decreased gradually. After low temperature treatment, the expression level of the BjuWRKY71s was increased significantly. BjuWRKY714 was the most responsive to the abiotic stresses. These results could provide a foundation on further explore the function of BjuWRKY71s in B. juncea tolerance to abiotic stress.

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YUAN Yuhui, WANG Kehong, DONG Haoran, SONG Bowen, LIU Xianjun. Identification and Expression Analysis of WRKY71 Transcription Factor Family Genes in Brassica juncea[J]. Acta Botanica Boreali-Occidentalia Sinica,2018,38(10):1801-1808

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  • Online: November 23,2018
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