Microbial lignin peroxidase is considered one of the key enzymes for lignin degradation. The lignin peroxidase gene (PcLIPH8) from Phanerochaete chrysosporium was introduced into plant binary expression vector for wildtype rice (Kitaake) transformation. Transgenic rice lines were confirmed by molecular identification. Enzymatic activity, lignin content determination and phenotypic changes were analyzed. The results showed that: (1) 35S∷PcLIPH8 plant binary expression vector was successfully transformed and three independent homozygous transgenic lines of rice were obtained. At seedling stage, there is no significant difference between transgenic rice lines and wildtype control. (2) Enzymatic activity and lignin content determination showed that lignin peroxidase activity increased 3.06%-5.07% and the lignin content decreased 11.44%-14.97% at seedling stage and 13.83%-20.05% at mature stage in transgenic rice lines compared with the wildtype control. (3) Phenotypic analysis at harvest showed that the plant height increased 28.37%-39.78%, panicle grain number increased 110%-120%, and biomass increased 18.61%-22.97% compared with the wildtype control. However, the grain weight decreased 12.86%-13.34%, and the grain length decreased 6.67%-7.15%. The research laid a foundation for the further use of the PcLIPH8 gene to reduce lignin content and increase biological production for improving plant quality.