In this study, Picea wilsonii was used as experimental material to clone a NAC transcription factor from cDNA library by PCR, which was named PwNAC30. Bioinformatics analysis showed that PwNAC30 open reading frame was 1 179 bp, encoding a total of 392 amino acids, a conserved NAM (no apical meristem) domain at its Nterminus and can be divided into five subdomains of AE. Multisequence comparison and phylogenetic tree analysis showed that PwNAC30 protein was clustered with North American spruce (P. sitchensis) of the Picea. Promoter cloning analysis showed that there were hormones and stress response elements such as gibberellin (GA), abscisic acid (ABA), methyl jasmonate (MeJA), TCrich repeats on the PwNAC30 promoter. Its promoter activity was significantly enhanced under ABA, GA, MeJA, low temperature, drought and salt treatments. Realtime PCR analysis showed that PwNAC30 had the highest expression in cones and the lowest in pollens and seeds. PwNAC30 responded to salt, drought, low temperature, ABA, MeJA, GA treatments, especially for salt, drought and MeJA. Subcellular localization experiments showed that PwNAC30 protein is localized in the nucleus and cytoplasm, mainly in the nucleus. Yeast one hybrid and two hybrid experiments showed that PwNAC30 protein had no transcriptional activation activity at its full length and Nterminus, whereas its Cterminus had transcriptional activation activity. PwNAC30 can form homodimers by itself. Studies have shown that PwNAC30 is a transcription factor with transcriptional activation activity at the Cterminus and is capable of forming a homodimeric structure by itself. It is widely involved in the signaling pathways of ABA, GA, MeJA and other hormones, and responds to salt, drought and low temperature.