Cloning and Expression Analysis of CsGGDPS7 Gene in Tea Plant (Camellia sinensis)
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    Abstract:

    In this study, geranylgeranyl diphosphate synthase 7 (CsGGDPS7) gene was identified from the tea genome. The fulllength cDNA of the CsGGDPS7 was cloned from the leaves of tea cultivar ‘Tieguanyin’ (GenBank accession number MH891780). The full length cDNA of CsGGDPS7 was 1 185 bp, with a 1 098 bp ORF, encoding 365 amino acids. Prediction results showed that CsGGDPS7 containing a Nterminal chloroplast targeting transit peptides, subcellular localization predicted that it could be located in chloroplast. Amino acid sequence analysis showed that the CsGGDPS7 had 5 conserved domains and 2 functional domains (DDXXXXD and DDXXD) of IsoprenoidBiosynC1 superfamily. Phylogenetic tree analysis showed that CsGGDPS7 and Olea europaea OeGGDPS7 had the closest genetic relationship. According to qPCR detection, expression of CsGGDPS7 in leaves was significantly higher than that of other tissues. There was no significant difference in the expression of CsGGDPS7 during the flower development. During the greenmaking procedure of oolong tea, the expression of CsGGDPS7 was significant upregulated rapidly from solar withering and reached its peak in the third tumbling. The expression of CsGGDPS1 and CsGGDPS2 were modulated by greenmaking procedure, but CsGGDPS4 did not.The result speculated that CsGGDPS7 might be related to the terpene aroma formation of oolong tea.

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WANG Zan, HUANG Xujian, CAO Hongli, YUE Chuan, GUO Yaling. Cloning and Expression Analysis of CsGGDPS7 Gene in Tea Plant (Camellia sinensis)[J]. Acta Botanica Boreali-Occidentalia Sinica,2019,39(1):32-41

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  • Online: February 28,2019
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