In order to understand the allelic variation and distribution of preharvest sprouting (PHS) genes in whet verieties of different regions, we genotyped 446 wheat cultivars from 7 wheat regions by the functional markers Vp1B3 and Dorm1 related to PHS resistance in China. The results indicated that: (1) Vp1 had three allelic variations detected by the marker Vp1B3: Vp1Bc (related to resistant PHS), Vp1Bb (related to resistant PHS) and Vp1Ba (related to susceptible PHS), and their frequencies were 62.8%, 4.3% and 32.9%, respectively. (2) DormB1 had two kinds of specific bands detected by the marker Dorm1:468 bp (genotype of DormB1a, correlated with susceptible PHS) and 606 bp (genotype of DormB1b, correlated with resistant PHS) accounted for 98.6% and 1.4%, respectively. (3) The detection by marker Dorm1 showed wheat germplasms with the genotype of DormB were distributed in Huanghuai winter wheat region (HHWWR) and Yangtze River middlelower reaches winter wheat region (YMWWR), with the ratios of 5.1% and 1.9%, respectively. The results of Vp1B3 indicated that wheat germplasms with (Vp1Bc+Vp1Bb) genotype (combination) were mostly distributed in YMWWR and Southwestern winter wheat region (SWWWR ) with the ratios of 75.0% and 74.1%, respectively. (4) Through the comprehensive identification of Vp1B3 and Dorm1 markers, 6 accessions of Vp1 and DormB1 loci related to resistant PHS were screened out, which were ‘Shanmai 611’, ‘Zhengnong 19’, ‘Yu 49198’, ‘Xinyang 0913’, ‘XianyangDasui’ and ‘Xuzhou 8066’, respectively. These accessions could be used as reference parents for PHS resistance breeding.