To construct a highdensity genetic linkage map of crested wheatgrass is the basis of fine mapping for important QTLs such as resistance, quality and yield, as well as the markerassisted breeding research. In this research, with 202 individuals from F₂ segregation population of tetraploid hybrid crested wheatgrass and their parents as materials, we constructed a genetic linkage map of crested wheatgrass based on SRAP molecular marker technique and Join map 4.0 mapping software. The results revealed that: (1) a total of 22 SRAP suitable primers with good polymorphism, clear and stable bands were screened to make a wide range of PCR amplification on the genomic DNA of F₂ individuals. A total of 510 SRAP polymorphic markers were obtained, accounting for 88.2%. (2) The analysis of segregation distortion showed that the ratio of distorted markers was only 14.12%, which met the requirements of genetic mapping. (3) The genetic linkage map of SRAP molecular markers of crested wheatgrass was successfully constructed. The map consisted of 14 linkage groups, including 510 SRAP markers, and covered the whole genome of 1 912.9 cM. The map length of linkage groups ranged from 86.4 cM to 179.0 cM, and the average distance between markers is 3.75 cM. It is a highdensity genetic map.