The cultivar ‘Vladivostok’ which was easy to drop and cultivar ‘Berel’ which was not easy to drop were both used as test materials. Paraffin section technique was used to observe the anatomical structure of the carpopodium abscission zone. We determined the contents of endogenous hormones in the carpopodium abscission zone by high performance liquid chromatography, and also determined the active of cell wall related enzymes by enzyme linked immunosorbent assay (ELISA) technique. we analyzed changes of endogenous hormones and cell wallrelated enzyme activities in different Lonicera cearulea L. during the formation of the carpopodium abscission zone, and hope to reveal the relationship between endogenous hormones and cell wallrelated enzymes and their effects on fruit abscission. The results showed: (1) there were no obvious fracture in ‘Berel’ carpopodium abscission zone at the whole fruit growth period, but ‘Vladivostok’ carpopodium abscission zone was gradually disintegration when the fruit matured, ultimately. It relies on vascular bundle connected between fruit and carpopodium. (2) There was a significant difference in hormone levels and trends in carpopodium abscission zone between ‘Berel’ and ‘Vladivostok’. At maturity, the ABA and ETH contents of ‘Vladivostok’ were higher than that of ‘Berel’, while the IAA and ZT contents of ‘Vladivostok’ were lower than that of ‘Berel’; the ‘Vladivostok’ GA₃ content was lower than that of ‘Berel’ in veraison. (3) In ‘Vladivostok’, the activities of Cx and βglu increased during the fruit ripening period, and the activities of PG and PME reached the peak at lastveraison. In ‘Berel’, Cx activities did not change throughout the fruit growth period; By contrast the activities of PG, PME and βglu peaked in fruit early growth stage, then decreased when the fruit matured. (4) In ‘Vladivostok’ carpopodium abscission zone, there was significantly negative correlation between Cx and IAA/ABA (P<0.05). In ‘Berel’ carpopodium abscission zone, βglu activity was significantly negatively correlated with IAA/ABA (P<0.05); PME activity was extremely significantly positively correlated with ABA content (P<0.01). Studies indicate, during fruit ripening, the ‘Vladivostok’ fruit abscission was related with the increase of ABA content, the decrease of IAA content and change in Cx and PG enzyme activities. In addition, Cx activity is negatively regulated by the relative content ratio of IAA to ABA. Change in PME enzyme activity of ‘Berel’ cell walls can promote fruit abscission, and change in content of ABA positively regulate PME enzyme activity.