In this study, three SOD genes were cloned from Brassica campestris L. ssp. chinensis(L.) var. utilis Tsen et Lee by homologous cloning and bioinformatics were analyzed. The expression patterns of three SOD genes in different tissues and under low temperature stress were analyzed by qRTPCR. The results showed that: （1） the ORF of Cu/ZnSOD, FeSOD and MnSOD genes were obtained and named BclCZSD, BclFSD and BclMSD. Their sequence length were 459, 639 and 696 bp, encoding 152, 212 and 231 amino acids, respectively. (2) Bioinformatics analysis showed that all three proteins were stable hydrophilic proteins without transmembrane structure and signal peptide. The secondary structure of BclCZSD protein was mainly random coil and had two Cu/ZnSOD domains, BclFSD and BclMSD proteins were mainly alpha helix and had one Mn/FeSOD domain. Evolutionary analysis showed that BclCZSD and BclMSD proteins were first clustered in one branch with SOD protein in Brassica napus, and BclFSD protein was clustered in one branch with Brassica oleracea, Raphanus sativus, Brassica napus and Brassica rapa. (3) The results of qRTPCR showed that BclCZSD, BclFSD and BclMSD genes were expressed with different expression profile in the roots, stems, leaves and petioles. The expression level of the 3 genes increased first and then decreased with the extension of time under low temperature. These results provide a foundation for the low temperature response mechanism study of SOD genes in Brassica campestris L. ssp. chinensis(L.) var. utilis Tsen et Lee.