DELLA protein is a core component of GA signaling pathway, and belongs to the GRAS nuclear transcription regulator family. In the present study, we used the grapevine cv. ‘Wink’ as the test material to identify the precise sequences and predict the potential functions of VvRGL1 and VvRGL2 through gene cloning, promoter analysis, chromosomal location, gene protein structure and phylogenetic analysis. And the qRTPCR method was used to detect the temporal and spatial expression of VvRGL genes in the grape berry peel, berry flesh and seed (or seed area) induced by exogenous GA. Result showed that: (1) the chromosomal localization of VvRGL1/RGL2 were Chr14 and Chr7, the open reading frame (ORF) were 2 007 bp and 1 815 bp, and the number of amino acid were 668 and 604, respectively. Both of VvRGL1 and VvRGL2 contained a GRAS conserved domain, but did not have a DELLA domain, and were members of the GRAS transcription factor gene family, but not the DELLA subfamily. Gene structure analysis result showed that their gene structures were highly conserved with the DNA sequence of VvRGL1 was only one intron and two exons, while the DNA sequence of VvRGL2 had no intron and only one exon. Phylogenetic analysis showed that VvRGL1 was closely related to Arabidopsis and citrus, while VvRGL2 was closely related to strawberry and poplar. (2) The promoters of two genes both contained elements that were responsible for GA and endosperm development, suggesting that they might be involved in response to GA signaling and endosperm development. (3) The results of qRTPCR showed that exogenous GA treatment reduced the expression of two genes in grape peel and seed area, but upregulated their expression in the flesh of young fruit, suggesting that in the peel and seed areas, GA might participate in the regulation of grape seedless fruit development by inhibiting the expression of VvRGL1/RGL2. This study provides an important basis for further explore the function of VvRGL in GA signaling pathway and in seedless fruit development of grapevine.