In this paper, a MYB gene named BnMYB3 was isolated from ramie (Boehmeria nivea L.) by amplification of cDNA ends (RACE), whose GenBank accession number is MF741320.1. The sequence analysis showed that the fulllength cDNA of BnMYB3 contained 1 216 base pairs, with a coding region of 900 base pairs. This sequence encoded a protein of 299 amino acids in length with a molecular weight of 33.63 kD and a theoretical isoelectric point of 9.16. The protein contains two typical MYB domains which belong to R2R3MYB gene family. The 1 681 base pairs promoter sequence of BnMYB3 was cloned from the genome of ramie. By bioinformatics analysis, the promoter sequence contained several stressrelated cisacting elements, such as ABRE, GAREmotif, CGTCAmotif and TGACGmotif. Realtime quantitative PCR analysis showed that BnMYB3 was a constitutive gene, but the expression levels of BnMYB3 in stems and leaves were significantly higher than that in roots. Furthermore, BnMYB3 gene was apparently induced by cadmium, and the relative expression level of BnMYB3 increased dramatically with the prolongation of treatment time and the increasement of Cd²⁺ concentration.