CML is an important Ca²⁺ response protein. In order to investigate the effect of CML protein on selfincompatibility in Primula, we screened PmCML19 and PfCML19 genes from Primula maximowiczii and P. forbesii respectively. Fulllength cloning, bioinformatics analysis, subcellular localization and expression pattern analysis of the two genes were performed. The results showed as follow： (1) the full length of PmCML19 and PfCML19 genes were 435 bp, and the similarity of their amino acid was 80.56%. Both of them were acid hydrophilic proteins and had four EFhand motifs. Homology analysis showed that the closest relationship with PmCML19 and PfCML19 was AcCML19 of Actinidia chinensis, while the similarity of amino acid sequences between PmCML19, PfCML19 and AtCML40 were 44% and 46% respectively. (2) The results of subcellular localization indicated that both PmCML19 and PfCML19 genes were expressed in the nucleus and cell membrane. (3) Realtime fluorescence quantitative PCR results demonstrated that the expression of PmCML19 and PfCML19 in anther were much higher than that in pistil. And the expression of PmCML19 and PfCML19 in compatible pollination pistils was higher than that in incompatible pistils. Based on these results, we speculated that CML19 gene of P. maximowiczii and P. forbesii is closely correlated with selfincompatibility in Primula, which provides a new idea for studying the genetic mechanism of heterotypic selfincompatibility.