Abstract:1-2 true leaves of Chinese olive healthy seedlings as experimental materials, seedlings roots were dipped in different solutions. We studied the effects of two kinds of exogenous auxin Indole3butyric acid (IBA) and 1naphthylacetic acetic acid (NAA) at different concentrations (100, 300 and 500 μmol/L) and the processing time (1, 3, 5 h) on the growth of roots and shoots of Chinese olive seedlings. The method of membership grade function was used to carry on the comprehensive evaluation. Further more, we measured chlorophyll fluorescence characteristics of seedlings of the top five processing, to find the best treatment to promote growth of Chinese olive seedlings and provide a theoretical and technical reference for propagation of Chinese olive rootstock seedlings. Results showed that: (1) exogenous auxin could promote the growth of Chinese olive seedlings and improve root system architecture. Treatments with lowconcentration and highconcentration for shortdurations had some better performances. They could enhance the growth potential of Chinese olive seedlings. (2) According to the comprehensive effect of membership function analysis on the growth of Chinese olive seedlings, the top five treatments were N3001>N5001>N1005>N1001>I3003. The first four treatments were all treated with NAA, which average membership function values were all greater than 0.65. Results showed the promotion of plant height, stem diameter, aboveground biomass, total root length, total root surface area, main root length and lateral root number under NAA treatment were greater compared with IBA treatment. (3) The chlorophyll fluorescence characteristics showed that Y(Ⅱ) of Chinese olive seedlings treated with N1001 was the highest, which was 8.16% higher than that of the control, ETR was the highest, NPQ was the lowest and significantly lower than CK by 23.78% (P<0.05). Therefore, Chinese olive seedlings under N1001 treatment was optimal. That is Chinese olive seedling roots treated for one hour with 100 μmol/L NAA, which significantly promoted the plant growth, and improved the utilization rate of light energy and the photosynthetic ability.