In order to explore the function of LEC1 gene to abiotic stress response in wheat, we cloned a TaLEC1 gene from wheat using RTPCR combined with RACE technology. The expression patterns of TaLEC1 from different tissues of wheat and under different stress treatments were analyzed by realtime fluorescence quantitative PCR (qRTPCR). These would be provided a foundation for the drought, high temperature and high salt response mechanism study of LEC1 gene in wheat. The results showed that: (1) the fulllength cDNA sequence of TaLEC1 was successfully cloned. The length of cDNA sequence of TaLEC1 is 1 074 bp, contains a 741 bp open reading frame (ORF), with 23 bp in the 5′ UTR and 310 bp in the 3′ UTR. TaLEC1 was predicted to encode a 246 amino acid protein with typical CBFD_NFYB domain. (2) Real time quantitative PCR (qRTPCR) analysis showed that there were significant differences in the expression levels of TaLEC1 among different tissues and the highest expression was found in 10 d leaves. (3) TaLEC1 could be upregulated by plant hormone ABA and belongs to ABAdependent expression regulation pathway. (4) During PEG simulated drought stress, TaLEC1 was induced to upregulate expression within 0.5~1 h of stress treatment. The expression of TaLEC1 showed a stable upregulation trend during the whole 42 ℃ stress process and the expression level was sharply upregulated under 12 h and 48 h of stress treatments, to 52.8 and 34.5 times of that of the control, respectively. TaLEC1 was rapidly upregulated within 0.5 h of high salt stress treatments. These research have shown that TaLEC1 is involved in ABAdependent stress response in wheat, suggesting that it may play an important dehydration protection function in wheat tolerance to high temperature and osmotic stress.