To explore the transmission characters of chromosomes from Thinopyrum elongatum in the background of durum, The lines Du_No.2 and Du_No.4 derived from a cross between Tritileytrigia 8801 (AABBEE) and T. turgidum L. ssp. durum (AABB) were characterized by chromosomespecific molecular markers, genomic in situ hybridization (GISH), and nondenaturing fluorescence in situ hybridization (NDFISH). The results showed that: (1) the molecular markers could amplify the specific bands of 2E and 4E chromosomes of Th. elongatum from line Du_No.2 and Du_No.4, respectively. (2) The analysis of GISH and NDFISH showed that the lines Du_No.2 and Du_No.4 were respectively added with a 2E and 4E chromosome, indicating that the line Du_No.2 and Du_No.4 were durumTh. elongatum 2E and 4E monosomic addition lines, respectively. (3) The meiosis process of two lines showed that both lines have E chromosome segregation abnormalities in anaphase Ⅰ, Ⅱ and telophase Ⅱ, and the abnormal rates of lines Du_No.2 and Du_No.4 were 22.24% and 36.18%, respectively. (4) PCR analysis of the progeny of the two lines with the durum showed that the transmission rates of 2E and 4E chromosomes through male gametes were 4.41% and 2.17%, respectively, while the transmission rates through female gametes were zero, indicating that 2E and 4E chromosomes could be transmitted by male gametes in the background of durum, but not through female gametes. This study provided a basis for the establishment of a complete set of durumTh. elongatum disomic addition lines and substitution lines.