HY5(ELONGATED HYPOCOTYL5) is an important transcription factor in photomorphogenesis process. We have cloned a bZIP transcription factor gene named MaHY5 from grape hyacinth(Muscari armeniacum) and its GenBank accession number is MK281355. Bioinformatics analysis of MaHY5 gene showed that it contains a 438 bp ORF that encodes a protein of 145 amino acid with an estimated molecular weight of 16.1 kD and an isoelectric point of 9.78. MaHY5 protein was not predicted to have an Nterminal signal peptide or a transmembrane domain and it was predicated to be a hydrophilic protein that is localized to the nucleus. The protein contains an bZIP domain which is highly conserved in evolution process, analyzed by sequence alignment and phylogenetic analysis. The qRTPCR and HPLC result indicated that the expression of MaHY5 was general correlated with the mode of anthocyanin accumulation. They were both highly expressed in petals, especially in fully open flowers. Thus, MaHY5 may play a role in the process of anthocyanins accumulation in grape hyacinth.