In this study, the fulllength cDNAs of two potato plant phytosulfokine receptor genes StPSKR1 and StPSKR2 were cloned from potato “Desiree”, and their bioinformatic analysis and localization analysis were performed. These will contribute to study the roles of PSK signal in potato growth, development and response to biotic stress. The results showed that: (1) the fulllength cDNA fragment of StPSKR1 and StPSKR2 genes were obtained by homologous cloning and PCR amplification, then cloned into pGWB5 vector. The protein sequence translated from the sequenced nucleotide results was consistent with that got from the database, indicating successful cloning of the StPSKR1 and StPSKR2 genes. (2) StPSKR1 is located on chromosome 1 of potato. The fulllength cDNA is 3 042 bp, encoding 1 013 amino acids. The relative molecular mass of predicted protein is 112.16 kD, the theoretical isoelectric point is 6.27. StPSKR2 is located on chromosome 7, and the fulllength cDNA is 3 135 bp. 1 044 amino acids with a relative molecular weight of 114.99 kD and a theoretical isoelectric point of 6.19. (3) Bioinformatic analysis showed that both StPSKR1 and StPSKR2 belonged to transmembrane proteins, including extracellular LRR regions, transmembrane regions and intracellular kinase regions. (4) Subcellular localization analysis showed that StPSKR1 and StPSKR2 localized on the cell membrane.