In this study, the PAL genes of three Dracaena (D.cochinchinensis, D. cambodiana and ‘Aizong’) plants were cloned by homologous cloning using the DNA and total RNA. Bioinformatics of PAL gene were analyzed with DNAMAN and MEGA7 software. The purpose of this study is to explore the molecular identification methods of three Dracaena plants, and provide a theoretical basis for the identification of different Dragons blood sources. The result show that: (1) three 718 bp PAL gene segments were obtained from D.cochinchinensis, D. cambodiana and ‘Aizong’, respectively. The homology of this 3 PAL genes was 99.69%, and just only 5 base mutations in base sequence. Phylogenetic analysis showed that the PAL gene of three Dracaena plants clustered with Liliaceae plants. (2) PAL gene was expressed in roots, stems and leaves of three Dracaena plants by RTPCR analysis. The difference of PAL gene expression in different tissues of Dracaena plants is small, and higher in ‘Aizong’ and lower in D.cochinchinensis and D. cambodiana. (3) Gene sequencing showed that PAL gene sequences were completely consistent in the same species, and just only 5 base mutations among three Dracaena plants. The PAL genes of three Dracaena plants are highly conserved. (4) Molecular identification method for three Dracaena plants was established based on the mutation site. Further molecular identification results indicate that GCGGG base site for D.cochinchinensis, CTGGC for D. cambodiana and GTTTG for ‘Aizong’. The research of molecular identification method is beneficial to the traceability and quality control of dragon’s blood.