ABF transcription factor (TF) is a basic leucine zipper protein, which involved in the growth, development and stress regulation in plants. In this study, a DcABF1 gene encoding ABF transcription factor was cloned from the carrot cv. ‘Junchuanhong’. The amino acid sequences, phylogenetic evolutionary relationships and physicochemical properties of DcABF1 were analyzed. The relative expression levels of the DcABF1 gene under different abiotic stress treatments were detected by quantitative realtime PCR. Sequence analysis indicated that the length of DcABF1 gene was 1293 bp and encoded 430 amino acids. The relative molecular mass of DcABF1 and theoretical pI were 104.96 kD and 4.850, respectively. The sequence alignment and phylogenetic analysis of the DcABF1 with the ABF TFs from other plants showed that the DcABF1 had the closest relationship with ABF from durian. The prediction of protein functional domain revealed that DcABF1 protein contained 28.84% αhelix and 56.05% random coil. There were some cis elements in the region of DcABF1 promoter. Quantitative realtime PCR analysis indicated that the DcABF1 gene had response to high temperature, low temperature, and salt stress treatments. The expression levels of DcABF1 were upregulated under salt treatment and peaked at 4 h. The expression level trend of DcABF1 under high salt treatment was first increased and then decreased. However, the expression of DcABF1 gene was downregulated compared with the control under drought treatment.