Amomum villosum is a kind of selfpollinated plant, whose pistil stands higher than the stamen and it is a typical material for studying generative cell of Monocotyledoneae on molecular biology. To solve the difficulty of A. villosum pollination, the researcher observed development process to clarify the structural characteristics of its pollen by phytohistochemistry method and isolated generative cells to explore its pollination technology by osmotic shock method. This research shows that: (1) the particularity of pollen development of A. villosum begins with tetrad stage and callosewall is absent. The four small spores are separated and enclosed by a polysaccharide cell wall during the tetrad period. Mature pollen is 2celled and it stores abundant polysaccharide starches and lipids in cytoplasm of A. villosum. Pollen exine is composed of polysaccharide not sporopollen substances of carotene and carotenoid esters. (2) The pollen of A. villosum broken rate is up to 10.67% and release pollen inclusions that contain generative cells in 2% mannitol+5% acetone solution. The generative cells remain intact for about 30 minutes in the released pollen cytoplasm. The isolated hundreds generative cells of A. villosum were collected by micromanipulation and stored in liquid nitrogen tank after placed in centrifuge tube every day.