The VAL2 gene was cloned by using Lilium oriental hybrid ‘Sorbonne’as a material (hereinafter referred to as LoVAL2). Sequence analysis showed that the coding sequence of LoVAL2 was 2 793 bp long and LoVAL2 encoded 930 amino acids containing five domains: PHDL, B3, CW, ZnFlike and EAR motif. Phylogenetic analysis showed that LoVAL2 was closely related to Asparagus officinalis, the following were Zea mays and Dendrobium catenatum, Arabidopsis thaliana and other plants were far away. Realtime RTPCR expression analysis showed that LoVAL2 expressed in stem root, tender stem, upper leaf, lower leaf and floral organ; LoVAL2 expressed in four kinds of floral organs. The relatively high expression levels in the pistils and stamens occurred in 5 mm buds; afterwards, the expression levels were downregulated with the development of flower buds; then, the expression levels were significantly upregulated in the pistil of 20 mm buds and the stamen of the 25 mm buds, indicating that LoVAL2 may play an important role in the early and the subsequent development of the pistil and stamen. LoVAL2 was localized in the nucleus of tobacco epidermal cells, which was consistent with the nuclear localization characteristic of transcription factors.