Light quality and photoperiod can regulate the formation of underground storage organs such as fleshy roots during plant growth. In this test, ‘Superior Queen No.2’ cherry radish was used as the test material, and white light (W) was used as a control to study the redblue light ratio of 8R2B, 5R5B, and 2R8B (the ratio of red and blue light was 8∶2, 5∶5, 2∶8, respectively) and 8L/16D, 12L/12D, 16L/8D photoperiod (light/dark was 8 h/16 h, 12 h/12 h, 16 h/8 h, respectively) on the effects of the growth and quality of cherry radish, which is screening combinations of light and quality photoperiod suitable for cherry radish growth. The test results showed that: long light (16L/8D) W, 8R2B treatment significantly promoted cherry radish leaf growth, fleshy root size and rootshoot ratio, and long light W was beneficial to cherry radish to form enlarged fleshy roots faster; The content of polyphenols and antioxidant capacity of cherry radish roots under long light W and 8R2B treatments were not significantly different; long light W was beneficial to the accumulation of soluble sugar and soluble protein in fleshy roots of cherry radish, and long light 8R2B was beneficial to the accumulation of soluble proteins in fleshy roots. The study found that the appropriate combination of light quality and photoperiod can significantly promote cherry radish leaf growth, fleshy root enlargement, and accumulation of nutrients in the root, thereby obtaining good growth and nutritional quality. The combination of long light (16L/8D) and white light was the best.